Research on Spatially Adaptive High-Order Total Variation Model for Weak Fluorescence Image Restoration

摘要： Confocal laser scanning microscopy (CLSM) has emerged as one of the most advanced ?uorescence cell imaging techniques in the ?eld of biomedicine. However, ?uorescence cell imaging is limited by spatial blur and additive white noise induced by the excitation light. In this paper, a spatially adaptive high-order total variation (SA-HOTV) model for weak ?uorescence image restoration is proposed to conduct image restoration. The method consists of two steps: optimizing the deconvolution model of the ?uorescence image by the generalized Lagrange equation and alternating direction method of multipliers (ADMM); using spatially adaptive parameters to balance the image ?delity and the staircase e?ect. Finally, an comparison of SA-HOTV model and Richardson-Lucy model with total variation (RL-TV model) indicates that the proposed method can preserve the image details ultimately, reduce the staircase e?ect substantially and further upgrade the quality of the restored weak ?uorescence image.