研究目的
Developing a rapid and sensitive fluorometric aptamer-based assay for the detection of aflatoxin B1 (AFB1) in food and environmental samples.
研究成果
The described fluorometric method for detection of AFB1 combines fluorophore labeled aptamer as a probe and cDNA-modified gold nanoparticle as a quencher, showing potential in applications for food safety and environmental analysis.
研究不足
The preparation of DNA modified gold nanoparticles may cause some limitations in the assays, but the process can be further improved.
1：Experimental Design and Method Selection:
The assay uses a FAM-labeled anti-AFB1 aptamer and complementary DNA-modified gold nanoparticles (GNPs) for fluorescence quenching and recovery based on AFB1 presence.
2：Sample Selection and Data Sources:
AFB1 spiked into 50-fold diluted wine and 20-fold diluted beer was used to test the assay's applicability in complex sample matrices.
3：List of Experimental Equipment and Materials:
Includes FAM-labeled aptamer, thiolated complementary DNA, HAuCl4·3H2O, trisodium citrate dehydrate, mycotoxins, black 96-wells microplates, and a Synergy H1 microplate reader.
4：Experimental Procedures and Operational Workflow:
Synthesis of GNPs, preparation of cDNA modified GNPs, determination of AFB1 using the aptamer based assay, and specificity tests.
5：Data Analysis Methods:
Fluorescence intensity was measured to determine AFB1 concentration, with statistical analysis of the results.
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