研究目的
The effective application of nanoparticles in cancer theranostics is jeopardized by their aggregation in biological media, rapid degradation and clearance. The design of biomimetic nanoconstructs with enhanced colloidal stability and non-immunogenicity is therefore essential.
研究成果
TNHs represent an efficient biomimetic platform for future nanotheranostic applications, with biomimetic extracellular vesicle-lipid envelope, facilitated ZnO cellular uptake and potential therapeutic implications. The study demonstrates that ZnO NCs can be efficiently incorporated and biostabilized within the lipidic bilayer of cell-derived EVs, allowing their stabilization toward degradation and aggregation in biological media.
研究不足
The study primarily focuses on in vitro experiments, and the in vivo stability, biodistribution, and therapeutic efficacy of TNHs remain to be investigated. The potential immunogenicity of heterologous EVs and the scalability of the TNH production process are also areas for future optimization.
1：Experimental Design and Method Selection:
The study involved the synthesis of ZnO nanocrystals through a microwave-assisted solvothermal approach, functionalization with amino groups, and coupling with extracellular vesicles (EVs) extracted from KB cell culture supernatants. The coupling efficiency was optimized by varying parameters such as temperature, time, mixing method, and dispersing medium.
2：Sample Selection and Data Sources:
KB cell line was used for EV extraction. ZnO NCs were synthesized and functionalized for coupling with EVs.
3：List of Experimental Equipment and Materials:
Equipment included a microwave reactor, ultracentrifuge, TEM, FESEM, DLS, NTA, and fluorescence microscope. Materials included zinc acetate dihydrate, KOH, methanol, APTMS, Atto647-NHS ester, and various buffers.
4：Experimental Procedures and Operational Workflow:
The process involved ZnO NCs synthesis, functionalization, EV extraction, coupling of ZnO NCs with EVs, and characterization of the resulting TNHs. Biological tests included cytotoxicity assays and internalization studies.
5：Data Analysis Methods:
Data were analyzed using fluorescence microscopy for colocalization, flow cytometry for internalization rates, and statistical analysis with ANOVA for significance.
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