研究目的
Investigating the effects of plasma sterilization on Bacillus subtilis var. natto using pulsed laser ablation and low pressure RF plasma, comparing the effectiveness of bulk and powder targets.
研究成果
Bacillus subtilis var. natto can be sterilized using low pressure RF plasma and pulsed laser ablation plasma, with powder targets and PLA plasma showing higher effectiveness. The sterilization effect is determined by the density of high-energy metal atoms and ions.
研究不足
The study focused on Bacillus subtilis var. natto and used specific plasma conditions; results may vary with other microorganisms or conditions.
1：Experimental Design and Method Selection:
The study used pulsed laser ablation (PLA) and low pressure RF plasma for sterilization, comparing bulk and powder targets.
2：Sample Selection and Data Sources:
Bacillus subtilis var. natto was used as the sterilization subject.
3：List of Experimental Equipment and Materials:
A pulsed Nd:YAG laser and RF plasma system were used, with Ti bulk and powder targets.
4：Experimental Procedures and Operational Workflow:
The chamber was evacuated, Ar gas was introduced, and plasma was generated for sterilization.
5：Data Analysis Methods:
The number of colonies was counted to judge sterilization effectiveness, and electron density and temperature were measured by a Langmuir probe method.
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