研究目的
Determination of Ciprofloxacin in Fish by Surface-Enhanced Raman Scattering Using a Liquid-Liquid Self-Assembled Gold Nanofilm
研究成果
A novel SERS method for the determination of trace ciprofloxacin in fish meat samples was proposed using a gold nanofilm prepared by self-assembly on the interface by simple vortex mixing of gold colloids and n-hexane. The method showed excellent precision and accuracy with a linear relationship across the concentration range from 0.5 to 10 lg/mL and a detection limit of 0.19 lg/mL.
研究不足
The study focuses on the detection of ciprofloxacin in fish samples using a specific SERS method. The applicability to other antibiotics or matrices was not explored. The optimization of Au NPs particle size for larger SERS enhanced factors was not conducted.
1：Experimental Design and Method Selection:
The study employed surface-enhanced Raman scattering (SERS) for the detection of ciprofloxacin in fish samples using a self-assembled gold nanofilm.
2：Sample Selection and Data Sources:
Fish samples were treated according to the National Standard of China (GB/T21312-2007) involving pretreatment by extraction and purification.
3：List of Experimental Equipment and Materials:
Gold colloids were prepared using HAuCl4 and sodium citrate. SERS spectra were recorded using a Labram high resolution ultraviolet 800 confocal Raman spectrometer.
4：Experimental Procedures and Operational Workflow:
The gold nanofilm was synthesized by mixing gold colloids and n-hexane, followed by vortex mixing. The SERS detection was performed on the self-assembled gold nanofilm.
5：Data Analysis Methods:
The relationship between ciprofloxacin concentration and the intensity of its characteristic Raman peak was analyzed to establish a linear range and detection limit.
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