研究目的
Investigating the design of benzothiadiazole-based covalent organic framework (COF) to enhance two-photon absorption (TPA) performance for improving near-infrared excited fluorescence imaging.
研究成果
The study demonstrates that TPA-COF can serve as a promising candidate with efficient TPA performance by utilizing the unique crystalline feature of COF. The slipped structure, long-range crystal domain, and π-conjugation domain significantly improve the delocalization of π-electrons, leading to high dipole value and TPA activity. This work offers a pathway to overcome aggregation-caused quenching and acquire near-infrared two-photon excited COFs for future biomedical applications.
研究不足
The study is limited by the specific design and synthesis of the benzothiadiazole-based COF and its application in fluorescence imaging, which may not be directly applicable to other materials or imaging techniques.
1：Experimental Design and Method Selection:
The synthesis of TPA-COF was achieved by solvothermal reaction of benzothiadiazole-based aldehyde molecular chromophores with aniline building blocks.
2：Sample Selection and Data Sources:
The structure of TPA-COF was characterized by Fourier transform infrared (FT-IR) spectrum, CP/MAS 13C NMR spectrum, N2 adsorption-desorption at 77 K, SEM, TEM, and PXRD patterns.
3：List of Experimental Equipment and Materials:
Instruments used include FT-IR, CP/MAS 13C NMR, SEM, TEM, PXRD, and DLS measurements.
4：Experimental Procedures and Operational Workflow:
The TPA-COF was synthesized and characterized, followed by spectroscopic studies and two-photon fluorescence imaging in vitro and in vivo.
5：Data Analysis Methods:
The TPA cross-section was determined by femtosecond fluorescence measurement technique, and the band gap was measured by UV-vis diffuse reflectance spectroscopic (DRS).
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