研究目的
Investigating the influence of the unsaturation of hydrophobic tails in oligopeptide amphiphiles on their self-assembly behavior and the potential for generating singlet oxygen for cancer treatment.
研究成果
The study demonstrates that the unsaturation of hydrophobic tails in oligopeptide amphiphiles significantly influences their self-assembly into shape-specific nanostructures. The linoleic acid-bearing OPA3 can be oxidized to generate singlet oxygen, offering a novel approach for designing self-deliverable nanomaterials for cancer treatment.
研究不足
The study focuses on the self-assembly behavior and singlet oxygen generation capability of oligopeptide amphiphiles but does not extensively explore their in vivo therapeutic efficacy or potential side effects.
1：Experimental Design and Method Selection:
The study involved the synthesis of three oligopeptide amphiphiles (OPA1-3) with different degrees of unsaturation in their hydrophobic tails and investigated their self-assembly behavior in aqueous medium.
2：Sample Selection and Data Sources:
OPA1-3 were synthesized manually by Fmoc-based solid phase synthesis. OPA4 was obtained by oxidizing OPA3 with lipoxidase.
3：List of Experimental Equipment and Materials:
Materials included Fmoc-Arg(Pbf)-OH, Fmoc-Asp(OtBu)-OH, etc., and equipment included MALDI-TOF-MS, TEM, AFM, FT-IR, CD spectroscopy, XRD, and SAXS.
4：Experimental Procedures and Operational Workflow:
The self-assembly behavior was initiated by adjusting the pH of OPA solutions. The morphology of self-assembled nanostructures was examined by TEM and AFM. The generation of singlet oxygen was verified using DCFH-DA and SOSG.
5：Data Analysis Methods:
The critical aggregation concentration (CAC) was evaluated by fluorescence spectrometry. The secondary structure of self-assemblies was characterized by FT-IR, XRD, and CD spectroscopy.
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Fluorescence Spectrometer
LS 55
PerkinElmer Instruments
Recording the fluorescence emission spectra.
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UV-visible Spectrophotometer
UV-2550
Shimadzu
Measuring the UV-visible absorption spectra.
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MALDI-TOF-MS
autoflex speed
Measuring the molecular weights of OPAs.
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TEM
TECNAI G2 20
Imaging the morphology of self-assembled OPAs.
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AFM
SPM-9700HT
Imaging the morphology of self-assembled OPAs.
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FT-IR
Nexus 470
Characterizing the secondary structure of self-assembled OPAs.
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CD Spectrometer
ChirascaTM-plus
Applied Photophysics
Recording the CD spectra of self-assembled OPAs.
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X-ray Diffractometer
D 8 ADVANCE
Obtaining the XRD spectra of self-assembled OPAs.
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SAXS
Measuring the size of original self-assemblies in aqueous solution.
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