研究目的
Development of a new nanocomposite optical sensor based on polyurethane foam and gold nanorods for solid-phase spectroscopic determination of catecholamines.
研究成果
The developed nanocomposite material and method allow for the sensitive and selective determination of catecholamines with low detection limits. The method is simple, cost-effective, and can be applied to the analysis of medicines.
研究不足
The method is limited by the potential aggregation of AuNRs at high concentrations of catecholamines, which can affect the accuracy of the determination. Additionally, the method requires a relatively long interaction time (30 min) for optimal results.
1：Experimental Design and Method Selection:
The study involved the synthesis of a nanocomposite material based on gold nanorods and polyurethane foam, and its modification with silver nitrate for the determination of catecholamines. The method is based on measuring the hypsochromic shift of a short-wave surface plasmon resonance band in the diffuse reflection spectrum of the nanocomposite.
2：Sample Selection and Data Sources:
Catecholamines (dobutamine, epinephrine, norepinephrine, and dopamine) were used as subjects of the study. The stock solutions were prepared by dissolving weighed portions in deionized water.
3：List of Experimental Equipment and Materials:
Instruments used included a diffuse reflection spectrophotometer, scanning electron microscope, transmission electron microscope, and zeta potential analyzer. Materials included hydrogen tetrachloroaurate, silver nitrate, sodium borohydride, ascorbic acid, cetyltrimetylammonium bromide, and polyurethane foam.
4：Experimental Procedures and Operational Workflow:
The nanocomposite was modified with silver nitrate and then interacted with catecholamines in glycine buffer solution. The interaction was monitored by measuring changes in the diffuse reflection spectrum.
5：Data Analysis Methods:
The shift of the short-wave maximum in the LSPR spectrum was used as an analytical response for the determination of catecholamines.
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Libra 200 transmission electron microscope
Libra 200
Carl Zeiss
Recording TEM-images of the samples
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JSM 7100 F scanning electron microscope
JSM 7100 F
Jeol
Investigation of the nanocomposites microstructure
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Eye-One Pro mini-spectrophotometer
X-Rite
Recording diffuse reflection in the visible region
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Formvar/Carbon Reinforced Copper Grids
3440C-MB
SPI
Deposition of dispersions of the samples in hexane/heptane
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Brookhaven Nano Brook Omni
Brookhaven
Zeta potential registration
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FDTD Solutions
Lumerical Inc.
Performing the calculations based on the finite-difference time-domain method
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Tswett Yauza chromatograph
NPO Khimavtomatika
Chromatographic determination of epinephrine and dopamine
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Luna С18 chromatographic column
30 × 2 mm
Used in chromatographic determination
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Millipore Simplicity
Millipore
Obtaining deionized water
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