研究目的
Investigating the potential therapeutic effects of cornel iridoid glycoside (CIG) on vascular dementia (VaD) in rats modeled by permanent bilateral common carotid artery ligation (2-vessel occlusion, 2VO).
研究成果
CIG ameliorated learning and memory impairment induced by chronic cerebral hypoperfusion in 2VO rats by increasing the expression of neurotrophic factors and glutamate receptors, and activating the PI3K/Akt/GSK3β/CREB signaling pathway. These results suggest that CIG may be a potential candidate drug for VaD therapy.
研究不足
The study was conducted on a rat model, which may not fully replicate human VaD conditions. The ameliorative effect of nimodipine was not significant in 2VO rats, suggesting potential limitations in translating findings to all therapeutic contexts.
1：Experimental Design and Method Selection
The study utilized the 2VO rat model to mimic chronic cerebral hypoperfusion in VaD. The object recognition test (ORT) and Morris water maze (MWM) test were conducted to evaluate learning and memory function. Western blot analysis and immunohistochemical staining were used to detect the expression of related proteins.
2：Sample Selection and Data Sources
Adult male Sprague-Dawley rats weighing 280-300 g (8 weeks old) were used. Rats were randomly assigned into 6 groups: sham, model, CIG-L (30 mg/kg), CIG-M (60 mg/kg), CIG-H (120 mg/kg), and nimodipine (16 mg/kg) groups.
3：List of Experimental Equipment and Materials
Equipment included a Leica cryostat for tissue slicing, Olympus microscope for imaging, and a computerized video system for MWM test analysis. Materials included primary antibodies for western blot and immunohistochemical staining, and CIG extracted from Cornus officinalis.
4：Experimental Procedures and Operational Workflow
Rats underwent 2VO surgery to induce chronic cerebral hypoperfusion. CIG was administered intragastrically for 3 months. Behavioral tests (ORT and MWM) were conducted at 1 and 3 months post-surgery. Brain tissues were then collected for protein expression analysis.
5：Data Analysis Methods
Data were analyzed using One-way Analysis of Variance with SPSS 16.0 software. Multiple comparison post-hoc tests between groups were performed with Least-Significant Difference test.
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