研究目的
To develop a grating-coupled Surface Plasmon Resonance (GC-SPR) sensor for label-free, real-time monitoring of living cells, focusing on cell adhesion kinetics and response to external stimuli.
研究成果
The GC-SPR system demonstrated effective real-time, label-free monitoring of cell adhesion and response to stimuli, offering a compact and simple alternative to traditional SPR systems. It holds promise for further biomedical research applications, despite some limitations in cell type compatibility and commercial availability.
研究不足
The study notes that some cell types may be sensitive to the grating structure, affecting cell shape, and the technique's commercial implementation is limited.
1：Experimental Design and Method Selection:
The study utilized a GC-SPR sensor with phase interrogation for real-time monitoring of cell adhesion and response.
2：Sample Selection and Data Sources:
SHI-1, a human acute myeloid leukemia (AML) cell line, was used to study cell adhesion dynamics.
3：List of Experimental Equipment and Materials:
The setup included a custom-made bench setup for SPR generation and detection, PDMS microfluidic chambers, and fibronectin for cell coating.
4：Experimental Procedures and Operational Workflow:
Cells were seeded on fibronectin-coated gold gratings, and their adhesion was monitored in real-time. Cell detachment was induced with trypsin to study dynamic responses.
5：Data Analysis Methods:
Reflectance spectra were analyzed to determine phase shifts proportional to refractive index variations, indicating cell adhesion and detachment.
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