研究目的
To develop a novel nanophotosensitizer through the covalent assembly of dopamine and genipin for combined photodynamic therapy (PDT) and chemotherapy, enhancing cancer treatment efficacy.
研究成果
The covalent assembly of dopamine and genipin resulted in nanoparticles (DGNPs) that serve as intrinsic photosensitizers for PDT and pH-responsive nanocarriers for chemotherapy. The DGNPs-Btz complex showed enhanced anticancer efficacy through combined therapy, demonstrating the potential of dopamine-based materials in cancer treatment.
研究不足
The study is preliminary, and further in vivo studies are needed to confirm the therapeutic efficacy and biocompatibility of DGNPs. The mechanism of 1O2 generation by DGNPs requires deeper investigation.
1：Experimental Design and Method Selection:
The study involved the one-step covalent assembly of dopamine and genipin to form nanoparticles (DGNPs). The size of DGNPs was modulated by varying the molar ratios and concentrations of the components.
2：Sample Selection and Data Sources:
Dopamine and genipin were used as primary materials. The anticancer drug bortezomib (Btz) was loaded onto DGNPs via pH-sensitive boronate esters.
3：List of Experimental Equipment and Materials:
TEM, SEM, DLS, FTIR, UV-Vis spectra, EPR, CLSM, and CCK-8 assay were used for characterization and evaluation.
4：Experimental Procedures and Operational Workflow:
DGNPs were synthesized, characterized, and their photosensitivity and drug release behavior were evaluated. The anticancer efficiency was tested on HeLa cells.
5：Data Analysis Methods:
The data were analyzed using various spectroscopic and microscopic techniques to confirm the formation, properties, and efficacy of DGNPs.
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TEM
Characterization of nanoparticle morphology
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SEM
Characterization of nanoparticle morphology
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DLS
Measurement of nanoparticle size distribution
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FTIR
Analysis of chemical bonds in nanoparticles
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UV-Vis spectra
Analysis of optical properties of nanoparticles
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EPR
Detection of singlet oxygen generation
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CLSM
Visualization of nanoparticle internalization in cells
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CCK-8 assay
Evaluation of cell viability
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