研究目的
To evaluate acute skin perfusion dynamics following thermal stimuli in healthy human volunteers and to examine potential mechanisms underlying tissue protection by local hypothermia or local hyperthermia.
研究成果
Despite higher oxygen consumption of tissue at higher temperatures, both blood flow and SO2 increase. Thus, we hypothesize that not only hypothermia but also hyperthermia may provide tissue protection.
研究不足
The study design limits the determination of effects of local cooling and warming belonging to cellular effects like cell death or distribution of proinflammatory cytokines. The study was restricted to non-invasive measuring of oxygen saturation and blood flow in healthy volunteers with intact skin. The range of temperature used may not lead to harmful effects to volunteers. For better understanding of these effects and to address tissue damaged caused by thermal influence, more precise examination with invasive measurements are needed.
1：Experimental Design and Method Selection:
The study used a TSA-II-NeuroSensory Analyzer to induce local hypothermia and hyperthermia at the palmar forearm of healthy volunteers. Changes in tissue microcirculation were assessed using an O2C device.
2：Sample Selection and Data Sources:
54 healthy volunteers were included, with exclusion criteria being cardiovascular diseases, diabetes mellitus, arterial hypertension, and skin diseases.
3：List of Experimental Equipment and Materials:
TSA-II-NeuroSensory Analyzer (Medoc Ltd., Ramat Yishai, Israel) and O2C device (Oxygen to See; LEA Medizintechnik, Gie?en, Germany).
4：Experimental Procedures and Operational Workflow:
Each subject was seated in a temperature-controlled room for at least 30 min. The forearm was cooled to 15°C and then to 5°C, and later heated to 40°C and then to 45°C. Microcirculation was assessed before and after each temperature change.
5：Data Analysis Methods:
The Shapiro-Wilk test was used to test for normality. The Friedman test was used to test for differences between groups, and the Mann-Whitney U test was performed for comparing two related samples. Spearman's rank correlation test was conducted to establish the association between two ranked variables.
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