研究目的
The aim of this study is to take advantage of the previous achievements and to develop an original strategy for the synthesis of a new class of hybrid nanostructures, namely plasmonic magneto-liposomes, by decorating cationic liposomes incorporating hydrophobic superparamagnetic iron oxide nanoparticles (SPIONs) in the bilayer with spherical hydrophilic gold nanoparticles.
研究成果
The synthesis of a new type of multifunctional magneto-plasmonic nanohybrid that could have a major impact in biomedical applications related to targeted drug delivery and release applications is reported for the first time. By synergistically employing and controlling hydrophobic and electrostatic interactions acting between the major building blocks, the synthesis of this new type of multifunctional nanohybrid was realized in a very reproducible manner. Each component of the nanohybrids was thoroughly characterized by means of complementary techniques, which allowed for the understanding of their properties. Once synthesized, the multifunctional magneto-plasmonic nanohybrids were characterized using the same techniques in order to evaluate the evolution of the individual properties in this complex system. The successful incorporation of superparamagnetic iron oxide nanoparticles, together with the modifications they generated in the lipid bilayer, were analyzed for the first time by means of Raman spectroscopy performed on dry samples, whereas the plasmonic properties of the hybrids were evaluated in solutions. Based on the properties here presented, we may estimate that the synthesis of this new type of multifunctional nanohybrids could represent a major breakthrough in biomedical applications.
研究不足
The technical and application constraints of the experiments, as well as potential areas for optimization, are not explicitly mentioned in the provided text.
The methodology for designing the experiment includes the synthesis of hydrophobic superparamagnetic iron oxide nanoparticles (SPIONs) and polyethylene glycol functionalized (PEGylated) gold nanoparticles (AuNPs). The SPIONs were incorporated into the liposomal lipidic bilayers to form cationic magnetoliposomes. Different concentrations of SPIONs were loaded in the membrane. The cationic magnetoliposomes were decorated with anionic PEGylated gold nanoparticles using electrostatic interactions. The successful incorporation of SPIONs and the modifications they generate in the bilayer were analyzed using Raman spectroscopy. The plasmonic properties of the multifunctional nanohybrids were investigated using UV-Vis absorption and (surface-enhanced) Raman spectroscopy. Their hyperthermic properties were recorded at different frequencies and magnetic field intensities.
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Tetramethylammonium hydroxide solution
25 wt.% in H2O
Sigma-Aldrich
Used in the hydrophilization step of SPIONs.
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Tetrachloroauric (III) acid
Sigma-Aldrich
Used in the synthesis of PEGylated gold nanoparticles.
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Iron (III) acetylacetonate
Sigma-Aldrich
Used in the synthesis of superparamagnetic iron oxide nanoparticles.
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Oleic acid
Sigma-Aldrich
Used in the synthesis of superparamagnetic iron oxide nanoparticles.
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Oleylamine
Sigma-Aldrich
Used in the synthesis of superparamagnetic iron oxide nanoparticles.
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1,2-hexadecanediol
Sigma-Aldrich
Used in the synthesis of superparamagnetic iron oxide nanoparticles.
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Chloroform
Sigma-Aldrich
Used as a solvent in the synthesis of liposomal dispersions.
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Benzyl ether
Sigma-Aldrich
Used as a solvent in the synthesis of superparamagnetic iron oxide nanoparticles.
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Polyethylene glycol 1000
Carl Roth
Used as both reducing and capping agents in the synthesis of PEGylated gold nanoparticles.
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Trisodium citrate dihydrate
Carl Roth
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Methylene blue
Carl Roth
Used in SERS measurements.
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1,2-Dioleoyloxi-3-trimethylammonium-propane chloride
Lipoid GmbH
Used in the synthesis of cationic liposomes.
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Soybean phosphatidyl-choline
Lipoid GmbH
Used in the synthesis of cationic liposomes.
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Sodium hydroxide micropearls
Lach-Ner
Used in the synthesis of PEGylated gold nanoparticles.
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Double-distilled water
18.2 M?
Used as a solvent in various synthesis processes.
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T92+UV–VIS Spectrophotometer
PG Instruments
Used for UV-Vis absorption measurements.
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HT7700 Transmission Electron Microscope
Hitachi
Used for TEM and EDS measurements.
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Brucker D8 Advance diffractometer
Bruker
Used for X-ray diffraction measurements.
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Vascoγ nanoparticle analyzer
Cordouan Technologies
Used for photon correlation spectroscopy measurements.
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Wallisζ Zeta potential analyzer
Cordouan Technologies
Used for zeta potential measurements.
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