研究目的
To determine the species composition of a mixture of microalgae by using the temperature dependence of their laser-induced fluorescence (LIF).
研究成果
The temperature dependence of fluorescence intensity of chlorophyll a varies greatly between different species of microalgae, allowing for the determination of species composition in mixtures. The proposed mathematical model explains the relationship of chlorophyll a fluorescence intensity with temperature and shows potential for ecological monitoring.
研究不足
The study was limited to microalgae cultures grown under constant conditions, and the mutual influence of cells of different cultures on each other may cause nonlinear relationships in fluorescence intensity.
1：Experimental Design and Method Selection:
The study involved measuring the LIF spectra of microalgae cultures and their mixtures under controlled temperature conditions.
2：Sample Selection and Data Sources:
Microalgae cultures were selected from the 'Marine Biobank' resource center and prepared to a uniform cell concentration.
3：List of Experimental Equipment and Materials:
A Shamrock 303i spectrometer, a temperature-controlled cell holder (TDK) QPOD 2e, and a magnetic stirrer were used.
4：Experimental Procedures and Operational Workflow:
Samples were heated from 20 to 80 °C at a rate of 1 °C/min, and LIF spectra were measured.
5：Data Analysis Methods:
The data were processed to subtract dark current, reduce noise, and normalize fluorescence intensity to water Raman scattering.
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