研究目的
Developing a method for the rapid and simultaneous screening and quantification of multi-class antibiotic pollutants in water using a planar waveguide immunosensor.
研究成果
The planar waveguide immunosensor developed in this study is capable of simultaneous screening and quantification of multi-class antibiotic pollutants in water with high sensitivity and specificity. The method simplifies the pretreatment process and shortens detection time, making it suitable for practical environmental monitoring.
研究不足
The study focused on a limited number of antibiotics (14) and may not cover all potential pollutants. The method's applicability to other types of pollutants was not explored.
1：Experimental Design and Method Selection:
A planar waveguide immunosensor (PWI) was used for the detection of antibiotics. The method involved integrating screening and quantitative detection sites on the same immunochip.
2：Sample Selection and Data Sources:
Real water samples were collected and spiked with antibiotics for recovery experiments.
3：List of Experimental Equipment and Materials:
The PWI platform, immunochip, Cy5.5-labelled antibodies, and various chemicals for chip modification and buffer preparation were used.
4：5-labelled antibodies, and various chemicals for chip modification and buffer preparation were used.
Experimental Procedures and Operational Workflow:
4. Experimental Procedures and Operational Workflow: The immunoassay involved pre-incubation, interfacial reaction, fluorescence collection, and regeneration steps. A testing buffer was developed to eliminate matrix effects.
5：Data Analysis Methods:
A four-parameter logistic model was applied to fit the fluorescence signals for quantification.
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