研究目的
The objective of this research was to investigate the effects of spectral conditions of energy and luminous intensity that induced high photostimulation of Tetradesmus sp. for the synthesis of carotenoids and chlorophylls a and b, by cultivation in a vertical tubular PBR injected with CO2-enriched air.
研究成果
The color LED combination and intensity significantly affected the biomass, carotenoids and chlorophyll a and b production of the microalga Tetradesmus sp., which presented high growth rate when cultivated under the highest energy blue light range. The red:green light combination promoted the highest concentrations of total carotenoids and chlorophylls.
研究不足
The study was conducted without replicates in some experiments, which may affect the robustness of the results. Additionally, the specific response of Tetradesmus sp. to light conditions may vary under different environmental or operational conditions not explored in this study.
1：Experimental Design and Method Selection:
The cultivation of Tetradesmus sp. was carried out in a BG-11 medium in a vertical tubular photobioreactor (PBR) irradiated with different spectral combinations and intensities of LEDs.
2：Sample Selection and Data Sources:
Tetradesmus sp. was isolated from a sample obtained in the municipality of Matelandia, Paraná, located in southern Brazil.
3：List of Experimental Equipment and Materials:
LEDs emitting light with white; red; yellow; green and blue spectrum were used alone and/or combined.
4：Experimental Procedures and Operational Workflow:
The cultures were performed with a 24:00 h (light:dark) photoperiod, at room temperature (25 ± 3 °C) and pH of
5：6 ± Data Analysis Methods:
The biomass concentration was determined by the gravimetric method and correlated to optical density at a wavelength of 683 nm (OD683) for diluted samples. The concentration of pigment was calculated using specific equations.
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