研究目的
To synthesize nitrogen and oxygen co-decorated carbon quantum dots (CQDs) for enhanced DNA plasmid expression in E. coli bacteria.
研究成果
The synthesized nitrogen and oxygen co-decorated CQDs significantly increased the gene expression of DNA plasmid in E. coli with high efficacy and low toxicity, making them promising candidates for gene therapy applications.
研究不足
The study focuses on E. coli bacteria and may not directly apply to other cell types or in vivo systems. The optimal concentration of CQDs for transformation efficiency was identified, but higher concentrations showed toxicity.
1：Experimental Design and Method Selection:
A bottom-up hydrothermal route was used for the synthesis of CQDs using ammonium hydrogen citrate (AHC) as a single precursor.
2：Sample Selection and Data Sources:
E. coli bacteria were used for transformation experiments with pTZ57R/T plasmid.
3：List of Experimental Equipment and Materials:
Ammonium hydrogen citrate, deionized water, Teflon-lined stainless autoclave, dialysis bag, spectrophotometer, spectrometer, TEM, XRD, XPS, FTIR, DLS.
4：Experimental Procedures and Operational Workflow:
Synthesis of CQDs, toxicity assay, transformation efficiency evaluation, fluorescence microscopy for uptake evaluation.
5：Data Analysis Methods:
Colony counting, fluorescence microscopy, gel electrophoresis for plasmid extraction and PCR analysis.
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Spectrophotometer
Avaspec 2048 TEC
Avantes
Used for recording photoluminescence spectra of CQDs.
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FTIR spectroscopy
Spectrum RX I
PerkinElmer
Used for recording the FTIR spectra of CQDs.
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Dynamic light scattering
ZEN 3600
Malvern
Used for obtaining the DLS spectrum of CQDs.
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Ammonium hydrogen citrate
Merck
Used as a single precursor for the synthesis of CQDs.
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Teflon-lined stainless autoclave
Used for hydrothermal synthesis of CQDs.
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Dialysis bag
1000 Dalton
Used for purification of CQDs.
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Spectrometer
Rey Leigh UV-2601
Used for recording ultraviolet-visible spectra of CQDs.
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Transmission electron microscopy
EM 208
Philips
Used for studying the shape and morphology of CQDs.
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X-ray diffraction
PW 1840
Philips
Used for obtaining the XRD pattern of CQDs.
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XPS spectroscopy
Used for studying the chemical composition of CQDs.
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