摘要： Theoretical and experimental approaches verifying the fluidic operation of a partially shielded digital microfluidics device are presented in this paper. This paper is motivated by recent demand from the synthetic biology community for electrowetting on dielectric (EWD) enabled in-droplet electroporation, but is generalizable to a range of EWD applications that require shielding structures to be patterned on the EWD. An electrode patterned in an additional metal layer on the insulator that supports EWD actuation reduces the effective strength of the EW force due to dielectric shielding at the droplet contact line. A numerical model was developed to predict the impact of the partially shielding electrode on threshold voltage, EW force, fluid velocity, and droplet transport time. Compared with a batch of devices lacking the extra electrode, the presence of the added metal layer resulted in a 29% increase in threshold voltage, an 82% increase in transport time, and a 44% decrease in average transport velocity. Each trend agrees with the simulation results obtained from the fluid transport model. These results support the development of design rules for microfluidic devices that require partially shielding metal layers to integrate with EWD device architectures.