研究目的
The aim of the current study was to explore the laser-induced fluorescence signatures in grapefruit plant leaves to evaluate the effect of citrus canker on its chlorophyll emission spectra. This might help in discriminating the citrus canker infected diseased leaves from healthy ones and it may ultimately assist in early detection of disease.
研究成果
The attack of citrus canker disease on grapefruit plant leaves mainly affects the chlorophyll emission band at 735 nm, which is produced in plant leaves through photosystem PSI, a pigment-protein complex involved in the initial stages of photosynthesis. In addition, the commencing of disease also increases the phenolic compounds which appeared at 530 nm. These two spectroscopic parameters form the basis for the detection of early onset of citrus canker disease. Therefore, the emission bands at 530 for phenolic compounds and 735 nm for chlorophyll may be considered as biomarkers for the detection of onset of disease. Both statistical techniques PCA and PLSR have successfully classified the healthy and diseased sites on plant leaves for the demonstration of early disease detection.
研究不足
The study focused on the detection of citrus canker disease on grapefruit plant leaves using fluorescence spectroscopy. The limitations include the specificity of the disease and plant type, and the need for further validation in different environmental conditions and with other plant diseases.
