研究目的
To develop an ultrasensitive, photoelectrochemical (PEC) detection of t-tau proteins using an artificial electron donor-free, bismuth vanadate (BiVO4)-based sensing platform.
研究成果
The incorporation of extrinsic Mo dopants and the FeOOH ad-layer into the BiVO4 photoelectrode and the amplification of photocurrent signals via HRP-catalyzed oxidation of DAB allowed the BiVO4-based PEC sensor to detect t-tau proteins down to femtomolar levels. The FeOOH/Mo:BiVO4-based PEC sensing platform accurately and selectively recognized t-tau proteins down to femtomolar concentrations with LOD and LOQ values of 1.59 fM and 4.11 fM, respectively.
研究不足
The study acknowledges that approximately over 15% of surface-reaching holes at FeOOH/Mo:BiVO4-Tau5 were not engaged in the water oxidation reaction, instead being lost to surface recombination, which was ascribed to the intrinsic limitations of FeOOH’s catalytic activity toward water oxidation reaction. Further studies to suppress the electron-hole recombination at water oxidation-coupled PEC platform are needed for accurate and reliable sensing of targeted biomarkers.
1：Experimental Design and Method Selection:
The study designed a FeOOH/Mo:BiVO4-based PEC sensing platform for detecting t-tau proteins. The methodology included the synthesis of pristine BiVO4 and FeOOH/Mo:BiVO4 photoelectrodes, characterization of these electrodes, and the fabrication of a Tau5 antibody-conjugated photoelectrode for PEC analysis.
2：Sample Selection and Data Sources:
Human tau441 was used as the sample, and the detection was performed in phosphate buffer saline (pH
3：4) and human plasma. List of Experimental Equipment and Materials:
Equipment included SEM, TEM, XPS, UV-Vis spectrophotometer, and electrochemical analyzers. Materials included Bi(NO3)3, vanadyl acetylacetonate, FeSO4, Tau5 antibody, HRP-labeled Tau46 antibody, and DAB.
4：Experimental Procedures and Operational Workflow:
The procedure involved the synthesis of photoelectrodes, conjugation of Tau5 antibody, exposure to t-tau proteins, and PEC analysis under LED illumination.
5：Data Analysis Methods:
Data were analyzed using linear sweep voltammetry, chronoamperometry, Mott-Schottky plots, and impedance spectroscopy.
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