摘要： Stimulus-responsive drug release possesses considerable significance in cancer precise therapy. Inspired by the continuous rotation-inversion movement of photoisomerizable azobenzene (Azo), this work designs an upconversion luminance fueled DNA-Azo nanopump for rapid and efficient drug release. The nanopump is constructed by assembling the Azo-functionalized DNA strands on upconversion nanoparticles (UCNPs). The selective intercalation of doxorubicin (DOX) in specific DNA helix leads to its efficient loading. Under near-infrared light, the UCNPs emit both UV and visible photons to fuel the continuous photo-isomerization of Azo, which acts as an impeller pump to trigger cyclic DNA hybridization and dehybridization for controllable DOX release. In a relatively short period, this system demonstrates 86.7% DOX release. By assembling HIV-1 TAT peptide and hyaluronic acid on the system, cancer cell nuclear targeting delivery is achieved for perinuclear aggregation of DOX and enhanced anticancer therapy. This highly effective drug delivery nanopump would contribute to chemotherapy development.