研究目的
Developing a simple and highly efficient strategy for preparing fluorescent hollow mesoporous carbon spheres (HMCSs) for drug delivery and cancer therapy, with a focus on improving the photothermal effect through 980-nm laser and microwave co-irradiation.
研究成果
The study successfully developed fluorescent HMCSs with low cytotoxicity and high drug loading capacity. The combination of 980-nm laser and microwave irradiation significantly enhanced the photothermal effect, leading to improved cancer therapy. HMCSs showed minimal toxicity and were effectively retained at the injection site, making them a promising candidate for clinical cancer therapy.
研究不足
The study primarily focuses on local administration and the effects on nasopharyngeal carcinoma. The applicability to other cancer types and systemic administration needs further investigation. Additionally, the long-term effects and potential for remote metastatic cancer cell killing require more research.
1：Experimental Design and Method Selection:
The study involved the preparation of HMCSs using a spray drying method followed by carbonization under nitrogen atmosphere. The methodology included evaluating the photothermal effect of HMCSs under 980-nm laser and microwave irradiation.
2：Sample Selection and Data Sources:
Nasopharyngeal carcinoma cells (CNE cells) and nude mice with CNE tumors were used as models. Data were collected through various assays including cell viability, tumor growth inhibition, and histological analysis.
3：List of Experimental Equipment and Materials:
Equipment included a spray drier, furnace, SEM, TEM, HRTEM, confocal fluorescence microscope, and microwave therapy instrument. Materials included bovine serum albumin (BSA), various drugs, and Pluronic F
4：Experimental Procedures and Operational Workflow:
1 The procedure involved the preparation of HMCSs, drug loading, in vitro and in vivo evaluation of photothermal effects, and toxicity assessment.
5：Data Analysis Methods:
Data were analyzed using statistical methods including one-way ANOVA to determine significant differences between groups.
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spray drier
Shanghai Da Chuanyuan Spray Drying Equipment Co., China
Used for spray drying aqueous BSA solution to prepare BSA particles.
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furnace
SLG1100-60
Shanghai Shengli Test Instruments Co. Ltd., Shanghai, China
Used for carbonization of BSA particles to prepare HMCSs.
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ultramicrotome
Leica Reichert Ultracut S Microtome StereoZoom 6
Wetzlar, Germany
Used for cutting embedded HMCSs into ultrathin sections for morphology evaluation.
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inverted fluorescence microscope
Nikon Eclipse TE2000
Japan
Used for imaging cells after incubation with HMCSs and irradiation.
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SpectraMax M5 universal microplate spectrophotometer
Molecular Devices Inc., CA, Sunnyvale, USA
Used for measuring luminescence intensities of cells.
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Microwave Therapy Instrument
SPW-1
Shengpu Medical Equipment Technology, Xuzhou, China
Used for exposing cells and tumors to microwaves to enhance photothermal effect.
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980-nm laser
Shanghai Inter-Diff Optoelectronics Technology Co., Ltd., Shanghai, China
Used for irradiating HMCSs to generate photothermal effect.
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thermal imager
Used for measuring temperature increases in tissues and solutions under irradiation.
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laser scanning confocal microscope
TCSNT
Leica, Nussloch, Germany
Used for imaging HMCSs and SCC-HMCSs.
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