研究目的
To compare the effects of ozone therapy (OT) and low level laser therapy (LLLT) on periodontal disease-induced bone destruction in rats with experimentally induced periodontitis (EP) by measuring HIF-1α, RANKL and OPG.
研究成果
Both OT and LLLT improve healing and reduce PMNL infiltration and hypoxia. OT reduced bone destruction, while LLLT increased bone formation compared to the control group.
研究不足
The principal limitation of the study was that OPG results were evaluated by staining scores; findings might be clarified by calculating the number of OPG cells and RANKL/OPG ratio in future studies.
1：Experimental Design and Method Selection:
The study used 30 male Wistar rats divided into three groups: control, OT and LLLT. EP was induced by placing a
2：0 silk suture around the cervix of the left mandibular first molar tooth. OT was performed using an ozone generator at 80% concentration. LLLT was applied using a diode laser. Both OT and LLLT were performed for two weeks at two day intervals. Sample Selection and Data Sources:
30 male Wistar rats (Rattus norvegicus albinus) were used.
3：List of Experimental Equipment and Materials:
Ozone generator (Ozone DTA generator; APOZA, New Taipei City, Taiwan), diode laser (CHEESE?; GIGAA, Wuhan, China),
4：0 silk suture. Experimental Procedures and Operational Workflow:
EP was induced, followed by OT and LLLT treatments. Histomorphometric and immunohistochemical analyses were performed.
5：Data Analysis Methods:
Statistical significance was calculated using IBM SPSS 20.0. The Kolmogorov-Smirnov test was used for adequate distribution. The Kruskal Wallis test was used to identify significant differences within experiments. Dunn’s multiple comparison post-test was used to assess the significance of differences between experimental groups.
独家科研数据包，助您复现前沿成果，加速创新突破
获取完整内容
