研究目的
To develop a digital controlled pulse generator for a possible tumor therapy combining irreversible electroporation with nanosecond pulse stimulation.
研究成果
The developed digital controlled high voltage pulse generator combining optic-coupler driver and silicon carbide MOSFET array is capable of delivering a complementary treatment on tumor cells, inducing apoptosis and antitumor immunity. It provides a convenient apparatus for further studies on this complimentary tumor therapy.
研究不足
The study acknowledges the challenge of balancing the stored energy during rising/falling edges of the pulse and the potential for current overflow and oscillation over a wide pulse range. The prototype's bulky common purposed DC voltage supplies could be optimized in future work.
1：Experimental Design and Method Selection:
The pulse generator was developed based on a capacitor discharging configuration digitally controlled by a silicon carbide MOSFET switching array through a pair of optic-coupler drivers.
2：Sample Selection and Data Sources:
Murine melanoma B16 cell lines and mice models were used for in-vitro and in-vivo studies.
3：List of Experimental Equipment and Materials:
Included a digital controlled pulse generator, silicon carbide MOSFET switching array, optic-coupler drivers, and various capacitors and resistors.
4：Experimental Procedures and Operational Workflow:
The pulse generator was tested on cell cultures and animal models to evaluate its effectiveness in inducing apoptosis and antitumor immunity.
5：Data Analysis Methods:
Flow cytometry was used to analyze cell apoptosis rates, and tumor sizes were measured to evaluate the in-vivo effects.
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