摘要： The bottom-up synthesis of graphene quantum dots (GQDs) using solvothermal method has attracted considerable attention because of their fewer defects and controllable size/ morphology. However, the influence of organic solvent on the preparation of GQDs is still unknown. Herein, a systematic study about carbonization of organic solvents toward GQDs is reported. Results show that organic solvent with the double bond or benzene ring or double hydrophilic groups could be directly decomposed into GQDs without the addition of catalysts or molecule precursors. The as-synthesized GQDs demonstrate ultra-small size distribution, high stability, and excitation wavelength-tunable and upconverted fluorescence. Both hematological and histopathological analyses studies show that the as-synthesized GQDs demonstrate a much good safety profile and excellent biocompatibility. The versatility of this synthetic strategy allows easy control of the surface group, composition, and optical properties of GQDs at the molecular level.