研究目的
To develop a novel ultrathin bismuthene-based sensing platform for microRNA (miRNA)-specific detection and to elucidate the fluorescence quenching mechanism of bismuthene.
研究成果
The study successfully developed a bismuthene-based biosensor for ultrasensitive detection of miRNA, with a detection limit of 60 pM. The fluorescence quenching mechanism was identified as ground state weakly fluorescent charge transfer. This platform holds promise for early cancer diagnosis and further exploration of bismuthene's applications in biosensing.
研究不足
The study is limited to in vitro experiments, and the applicability of the biosensor in intracellular environments needs further investigation.
1：Experimental Design and Method Selection:
The study involved the preparation of bismuthene nanosheets via liquid-phase sonication and their characterization using AFM, HRTEM, XRD, and Raman spectroscopy. The interaction between bismuthene and FAM-ssDNA was studied using steady-state and time-resolved measurements, including femtosecond pump-probe spectroscopy.
2：Sample Selection and Data Sources:
FAM-labeled ssDNA was used as the probe for miRNA detection. The samples were prepared with various concentrations of bismuthene and miRNA-
3：List of Experimental Equipment and Materials:
Atomic force microscopy (AFM), high-resolution transmission electron microscopy (HRTEM), X-ray diffraction (XRD), Raman spectroscopy, and femtosecond pump-probe spectroscopy were used.
4：Experimental Procedures and Operational Workflow:
The bismuthene nanosheets were prepared and characterized. The interaction between bismuthene and FAM-ssDNA was studied, and the fluorescence quenching mechanism was elucidated. The sensitivity and selectivity of the biosensor were tested with different concentrations of miRNA-
5：Data Analysis Methods:
The fluorescence spectra and transient absorption spectra were analyzed to understand the quenching mechanism and the performance of the biosensor.
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