研究目的
To develop an ultrasensitive electrochemical aptasensor for the determination of β-casomorphin 7 (BCM-7) as a promising biomarker of autism disorder.
研究成果
The developed aptasensor based on AuNPs@ZnS-QDs nanocomposite is ultrasensitive and selective for the detection of BCM-7, with potential applications in the early diagnosis of autism spectrum disorders.
研究不足
The study does not mention any specific limitations.
1：Experimental Design and Method Selection:
The study utilized an electrochemical aptasensor based on a glassy carbon electrode (GCE) modified with thiourea capped-ZnS QDs and gold nanoparticles (AuNPs) for the immobilization of an amino-aptamer (NH2-Apt).
2：Sample Selection and Data Sources:
The NH2-Apt sequence for distinguishing BCM-7 was purchased from Takapouzist Co. (Tehran, Iran).
3：List of Experimental Equipment and Materials:
A μ-Autolab Potentiostat/Galvanostat, a Metrohm model 780 pH/mV meter, a Vertex 70 FT-IR instrument, a Carry Eclipse fluorescence and phosphorescence spectrometer, and a TESCAN SEM were used.
4：Experimental Procedures and Operational Workflow:
The GCE was modified with ZnS-QDs and AuNPs, followed by the immobilization of NH2-Apt. The aptasensor was then used to detect BCM-7 in various concentrations.
5：Data Analysis Methods:
Cyclic voltammetry (CV), differential pulse voltammetry (DPV), and electrochemical impedance spectroscopy (EIS) were used to evaluate the aptasensor's performance.
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Metrohm model 780 pH/mV meter
780
Metrohm
Used for pH adjustment.
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Carry Eclipse fluorescence and phosphorescence spectrometer
Eclipse
Agilent
Used for obtaining fluorescence spectrum.
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μ-Autolab Potentiostat/Galvanostat
Eco-Chemie
Used for electrochemical measurements.
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Vertex 70 FT-IR instrument
70
Vertex
Used for recording FT-IR spectra.
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TESCAN SEM
TESCAN
Used for SEM imaging of the electrode surface.
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