研究目的
To measure the birefringence of biological tissues with complex structures using polarization-sensitive digital holography.
研究成果
The polarization-sensitive digital holography system successfully measures the birefringence of bladder tissues, showing distinct phase distributions and birefringence values between normal and cancerous tissues. The median birefringence value of cancerous tissue is significantly higher than that of normal tissue, providing a potential criterion for pathological research and clinical diagnoses.
研究不足
The study is limited to bladder tissue samples and may not be directly applicable to other types of biological tissues without further validation. The technique requires precise alignment and calibration of the optical system.
1：Experimental Design and Method Selection:
The study uses a modified Mach–Zehnder interferometer system to measure the birefringence of biological tissues. The system incorporates a polarization-sensitive digital holography technique to record polarization-dependent phase-shifted holograms.
2：Sample Selection and Data Sources:
Normal and cancerous bladder tissue samples were obtained from patients who had undergone radical cystectomy.
3：List of Experimental Equipment and Materials:
The system includes a MSL-FN-532 nm single longitudinal mode laser, CCD with a resolution of 2452 × 2054 pixels, quarter-wave plate (QWP), polarizer (P), beam splitters (BS1, BS2), mirrors (M1, M2), and 40 × (
4：45 NA) microscope objectives (MO1, MO2). Experimental Procedures and Operational Workflow:
The output beam of the laser is attenuated and turned into circular polarized light by the QWP. The polarization state is changed by rotating the P. The beam is split into object and reference waves, which interfere on the CCD sensor to generate off-axis digital holograms.
5：Data Analysis Methods:
The hybrid reconstruction algorithm and least-square unwrapping are used to extract phase information from the holograms.
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