1：Experimental Design and Method Selection:

The study involved the design and synthesis of glutamide-derived amphiphilic Schiff bases with different aldehyde moieties. The self-assembly behavior of these Schiff bases in acetonitrile was investigated to form supramolecular gels or precipitates. The effect of metal ions (Mg2+, Zn2+, Al3+) on the fluorescence and CPL properties of these Schiff bases was examined.

2：Sample Selection and Data Sources:

The samples were synthesized by refluxing N,N’-bis(octadecyl)-L-glutamic diamide (LG) with various aromatic aldehydes in ethanol. The self-assembled structures were characterized using SEM, XRD, UV-Vis, CD, and fluorescence spectroscopy.

3：List of Experimental Equipment and Materials:

Equipment included a Bruker AV 400 spectrometer, BIFLEIII MALDI-TOF-MS, Hitachi S-4800 FE-SEM, Rigaku D/ Max-2500 X-ray diffractometer, Hitachi UV-3900, JASCO J-1500 spectrometers, Hitachi F-4600 fluorescence spectrophotometer, and JASCO CPL-200 spectrometer.

4：Experimental Procedures and Operational Workflow:

The synthesis of Schiff bases involved refluxing LG with aldehydes in ethanol. The self-assembly was induced by dissolving the compounds in acetonitrile and cooling to room temperature. Metal ion coordination was achieved by adding metal ion solutions to the Schiff base solutions.

5：Data Analysis Methods:

The data were analyzed using various spectroscopic techniques to determine the structural, optical, and chiral properties of the self-assembled materials.