研究目的
To develop a multifunctional nanoplatform based on hyaluronic acid modified Au nanocages (AuNCs-HA) for enhanced photoacoustic imaging, photothermal therapy, radiotherapy, and photodynamic therapy, aiming at achieving synergistic therapeutic effects for tumor treatment.
研究成果
The developed AuNCs-HA nanoplatform exhibits significant potential for PA imaging-guided combined radiotherapy, PDT, and PTT, leading to effectively synergistic anticancer efficacy. This multifunctional approach offers a promising strategy for cancer theranostics, integrating imaging and therapeutic functionalities for enhanced tumor treatment.
研究不足
The study primarily focuses on the in vitro and in vivo evaluation of AuNCs-HA in murine models, and further clinical translation would require extensive preclinical and clinical trials to assess safety, efficacy, and potential side effects in humans.
1：Experimental Design and Method Selection:
The study involved the synthesis of AuNCs-HA through a galvanic replacement reaction between Ag nanocubes and HAuCl4, followed by modification with hyaluronic acid (HA) for targeted delivery. The nanoplatform's functionalities were evaluated for PA imaging, PTT, PDT, and RT.
2：Sample Selection and Data Sources:
Murine 4T1 breast cancer cells and tumor-bearing mice were used as models to assess the therapeutic efficacy and imaging capabilities of AuNCs-HA.
3：List of Experimental Equipment and Materials:
Gold(III) chloride trihydrate, polyethylene glycol (PEG), N-(3-Dimethylaminopropyl)-N-ethylcarbodiimide hydrochloride (EDC), N-Hydroxysuccinimide (NHS), ethylene glycol, and polyvinylpyrrolidone (PVP) were used among other reagents.
4：Experimental Procedures and Operational Workflow:
The synthesis of AuNCs-HA, characterization, in vitro and in vivo evaluations including cytotoxicity, ROS generation, photothermal effect, PA imaging, and therapeutic efficacy were systematically conducted.
5：Data Analysis Methods:
Data were analyzed using statistical methods including one-way ANOVA and student's two-tailed t tests to evaluate the significance of the experimental results.
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