研究目的
To discuss state-of-the-art developments in quantitative light microscopy in the context of C. elegans research and the impact these technologies have on the field.
研究成果
The review concludes that current imaging techniques are crucial for studying dynamic cellular processes in space and time but are limited by the number of biological components that can be recorded. It emphasizes the potential of combining sequencing-based single-cell technologies with high-resolution imaging to revolutionize biological research.
研究不足
The review highlights technical challenges such as the limited number of biological components that can be recorded in each experiment due to the limited size of the wavelength spectrum and available fluorophores. It also notes the difficulty in robustly assigning all cells over time in neuronal activity imaging.
