研究目的
Determining the minimum ultraviolet (UV) irradiation dose to influent water to prevent Kudoa yasunagai infection.
研究成果
The minimum effective UV irradiation dose required for complete prevention of K. yasunagai infection lies between 5 and 15 mJ/cm2. UV irradiation at doses normally used against bacteria and some viruses should be sufficient to control K. yasunagai and possibly other Kudoa species in land-based influent facilities.
研究不足
The influent water used in the study was pre-filtered by a drum filtration system, and the effective UV dose for raw seawater may be higher.
1：Experimental Design and Method Selection:
A laboratory experiment was conducted to determine the minimum UV irradiation dose for preventing K. yasunagai infection in Seriola lalandi.
2：Sample Selection and Data Sources:
Juvenile S. lalandi were reared in water treated with different UV doses (0, 5, 15, or 30 mJ/cm2).
3：2). List of Experimental Equipment and Materials:
3. List of Experimental Equipment and Materials: UV irradiator (PW10, Ebara Jistugyo Co., Ltd.), QIAmp DNA Mini Kit (Qiagen), Rotor-Gene 6000 system (Qiagen), and commercial pellets.
4：Experimental Procedures and Operational Workflow:
Fish were reared for 7 weeks in tanks with UV-treated water, sampled weekly, and examined for K. yasunagai infection by microscopy and qPCR.
5：Data Analysis Methods:
Statistical analyses were performed on SPSS 15.0 to compare infection prevalence between groups.
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