研究目的
To develop a sensitive colorimetric method for the detection of 6-thioguanine (6-TG) based on its protective effect on silver nanoprisms (AgNPRs) against iodide-induced etching, applicable for biomedical analysis.
研究成果
A sensitive and selective colorimetric method for 6-TG detection was developed, based on its protective effect on AgNPRs against iodide-induced etching. The method offers a simple, cost-effective solution for monitoring 6-TG levels in biological fluids, with potential applications in clinical settings.
研究不足
The method's selectivity may be affected by certain ions and compounds in complex biological samples, requiring careful sample preparation and analysis.
1：Experimental Design and Method Selection
The method is based on the protective effect of 6-TG on AgNPRs against iodide-induced etching, leading to a color change from blue to pink. The presence of 6-TG prevents this color change, allowing for its detection.
2：Sample Selection and Data Sources
Human plasma samples spiked with 6-TG were used to test the method's applicability in biomedical analysis.
3：List of Experimental Equipment and Materials
Cary-100 spectrophotometer (Varian, Australia), transmission electron microscopy (TEM, EM 900, Zeiss, Germany), Metrohm model 827 pH meter, AgNO3, trisodium citrate dehydrate, H2O2, NaBH4, KI, 6-TG, Poly(vinylpyrrolidone) (PVP, MW~ 58000 g mol-1).
4：Experimental Procedures and Operational Workflow
AgNPRs were synthesized and characterized. The detection of 6-TG was based on its protective effect against iodide-induced etching of AgNPRs, observed through color change and UV-vis absorption spectra.
5：Data Analysis Methods
The wavelength shift in the UV-vis absorption spectrum of AgNPRs in the presence of I- and 6-TG was used to quantify 6-TG concentration.
独家科研数据包，助您复现前沿成果，加速创新突破
获取完整内容
