研究目的
To explore the effect of NIR irradiation on lipid metabolism in neuronal cells.
研究成果
NIR light induces significant changes in lipid metabolism and LDs formation in neuronal cells, mediated by ROS. These findings contribute to the development of therapeutic approaches for neurodegenerative disorders based on the manipulation of cellular lipid metabolism with NIR light.
研究不足
The study focuses on the effects of NIR light on lipid metabolism in rat cortical neurons, and the findings may not be directly applicable to other cell types or in vivo conditions. The exact mechanisms of NIR light effects on lipid metabolism and LDs formation are not fully clarified.
1：Experimental Design and Method Selection:
Employed CARS microscopy for real-time monitoring and quantitative analysis of the NIR light effect on lipid metabolism and LDs formation in neuronal cells.
2：Sample Selection and Data Sources:
Primary Rat Cortex Neuron (RCN) cells were used, cultured in complete NeurobasalTM medium.
3：List of Experimental Equipment and Materials:
Diode laser (LU0808T020; Lumix, Germany), Leica SP2 confocal laser scanning microscope, HCS LipidTOX? Red fluorescence probe, dihydrorhodamine-
4：Experimental Procedures and Operational Workflow:
1 Cells were irradiated with NIR light at 808 nm with different doses, and changes in lipid content and LDs formation were monitored over time using CARS and fluorescence microscopy.
5：Data Analysis Methods:
ImageJ software was used for quantitative analysis of CARS signal intensity and LDs counting.
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