研究目的
To improve the sterilization effect and energy efficiency of atmospheric pressure non-equilibrium plasma (APNP) by combining it with photocatalysis to inactivate Escherichia coli.
研究成果
The APNP treatment combined with photocatalysis has a better effect on the sterilization than traditional APNP treatment with a maximum enhancement factor η of 80.4%. The study demonstrates that APNP combined with photocatalysis produces more ROS and has a better bactericidal effect, while also reducing the temperature rise and increasing the utilization of energy.
研究不足
The study focuses on the inactivation of Escherichia coli and may not be directly applicable to other bacteria or microorganisms. The experimental conditions, such as the flow rate of helium and the power supply frequency, are specific and may limit the generalizability of the findings.
1：Experimental Design and Method Selection:
A synergistic treatment system of APNP combined with photocatalysis was established. TiO2 was selected as the photocatalyst.
2：Sample Selection and Data Sources:
E. coli cells with ampicillin (AMP) resistant plasmids were seeded on the Luria–Bertani (LB) medium containing AMP in 35 mm diameter petri dishes.
3：List of Experimental Equipment and Materials:
The experimental setup consists of a power supply device, a plasma jet device, and a testing system. The power supply device (CTP-2000K) provides a sine high voltage. The voltage and current waveforms were obtained by a digital oscilloscope (Agilent Technologies DSO1024A), a high-voltage probe (Tektronix P6015A), and a current transformer (CT-C
4：5-SMA). Experimental Procedures and Operational Workflow:
The flow rate of helium was controlled at 15 ml/min. The power supply frequency was maintained at 7 kHz. The voltage and current waveforms of plasma were recorded.
5：Data Analysis Methods:
Colony counting and scanning electron microscope (SEM) were employed to demonstrate the bactericidal effect. The concentration of ROS was determined by ultraviolet spectrophotometry.
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