研究目的
To describe the theoretical aspects of wavelet transform methodologies and its typical implementations for UV spectroscopic analysis of pharmaceuticals.
研究成果
Continuous wavelet transform-based UV spectroscopic analysis methods have outperformed both conventional and derivative UV spectroscopy in resolving spectrally binary and ternary mixtures. However, wavelet analysis may not also have a sufficient power to resolve overlapping spectra of analytes in samples due to similarity of molecular structures and signal frequencies in some cases. They may not give desirable results for a complex mixture containing more than three compounds and/or a significant difference in ratios of active ingredients. In such a case, the use of WT coupled with chemometric PLS and PCR calibrations is advisable.
研究不足
Wavelet transform methods may not have sufficient power to resolve overlapping spectra of analytes in samples due to similarity of molecular structures and signal frequencies in some cases. They may not give desirable results for a complex mixture containing more than three compounds and/or a significant difference in ratios of active ingredients.
