研究目的
Investigating the ability to distinguish Escherichia coli cells from polystyrene beads using fluorescence imaging on a rotating optical disk.
研究成果
The study successfully demonstrated the construction of a fluorescence image from a rotating optical disk sample, distinguishing E. coli cells from polystyrene beads. This method shows promise as a sensor for detecting targets among contaminants on a large area.
研究不足
The study mentions technical limitations such as low signal-to-noise ratio and ghost noise in the fluorescence image, which could be improved by optimizing the fluorescent staining solution and eliminating sub-laser beams.
1：Experimental Design and Method Selection:
The study utilized a fluorescence imaging method on a rotating optical disk to distinguish E. coli cells from polystyrene beads.
2：Sample Selection and Data Sources:
E. coli cells and polystyrene beads were placed on the disk surface, and a cell staining solution was used.
3：List of Experimental Equipment and Materials:
Optical disk drive tester, laser, beam splitter, objective lenses, detectors, long-pass filters, and a signal processor were used.
4：Experimental Procedures and Operational Workflow:
The laser was scanned along the spiral groove of the disk substrate, and fluorescence was measured from the substances.
5：Data Analysis Methods:
The signal processor constructed an image by aligning the laser-scanned results based on reflected lights detected at positioning marks.
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