研究目的
To investigate the bactericidal efficacy of methylene blue (MB)-based photodynamic therapy (PDT) on Pseudomonas aeruginosa (P. aeruginosa), which is the major cause of drug resistant post-endoscopy outbreak, and to assess the synergistic effects of hydrogen peroxide addition to MB-based PDT on biofilms.
研究成果
MB-based PDT with hydrogen peroxide significantly enhances the bactericidal effect on P. aeruginosa biofilms, suggesting a potential application in endoscopic disinfection to prevent post-endoscopy outbreaks.
研究不足
The study was conducted in vitro, and the findings may not directly translate to clinical settings. The effect of MB-based PDT with hydrogen peroxide on other bacterial species or in more complex biofilm environments was not investigated.
1：Experimental Design and Method Selection:
The study evaluated the efficacy of MB-based PDT on P. aeruginosa in both planktonic and biofilm states, with and without hydrogen peroxide.
2：Sample Selection and Data Sources:
P. aeruginosa KCTC 2004 was used, cultured in Nutrient Broth.
3：List of Experimental Equipment and Materials:
Methylene blue (Junsei Chemical), hydrogen peroxide (Sigma-Aldrich), 300mW laser of 660nm wavelength, Nutrient Broth (BD Difco), Nutrient agar (BD Difco), LIVE/DEAD BacLight Bacterial Viability Kit (Invitrogen), Zeiss Axiovert 200 fluorescence microscope.
4：Experimental Procedures and Operational Workflow:
Bacterial suspensions were treated with MB at various concentrations, with or without hydrogen peroxide, followed by laser irradiation. Biofilm formation and viability were assessed using crystal violet assay and live/dead assays.
5：Data Analysis Methods:
Data were analyzed using SAS software, with statistical significance set at P < 0.05.
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