研究目的
To demonstrate a relatively simple means to produce a 3D infrared video of a strawberry plant freezing, allowing a more accurate identification of sites for ice nucleation as well as the progression of freezing.
研究成果
The 3D infrared video of plant freezing provides a more accurate identification of sites for ice nucleation and the progression of freezing, which can help in developing more freezing-tolerant strawberry genotypes and in preventing damage to plants during freezing conditions.
研究不足
The requirement of red-blue glasses to view the 3D video and the limited chromatic view provided by red-blue anaglyph videos.
1：Experimental Design and Method Selection:
The technique involves positioning two IR cameras at slightly different angles to film the strawberry freezing. The two video streams are precisely synchronized using a screen capture software that records both cameras simultaneously. The recordings are then imported into the imaging software and processed using an anaglyph technique.
2：Sample Selection and Data Sources:
A 6-weeks-old strawberry plant with 2 - 5 flowers grown in a 1 L container is used.
3：List of Experimental Equipment and Materials:
Two IR cameras (e.g., FLIR T620 cameras), a programmable freezer, a computer with screen capture and video processing software, and red-blue glasses.
4：Experimental Procedures and Operational Workflow:
The plant is placed in a freezer programmed to reach -8 °C at 1 °C/h. The IR cameras are set up to film the plant from slightly different angles, and the video streams are recorded and processed to produce a 3D video.
5：Data Analysis Methods:
The 3D video is analyzed to determine the precise site of ice nucleation on leaf surfaces.
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