研究目的
To demonstrate the light controlled guiding and injection of plasmonic Janus nanopens into living cells for applications in nano-surgery, sensing, and drug delivery.
研究成果
The study successfully demonstrates an all-optical strategy for injecting plasmonic Janus nanopens into living cells, enabling the controlled delivery of genetic material. The technique combines the benefits of optical trapping and thermophoretic forces for precise positioning and injection, with the dielectric part of the nanopen remaining functional for biomolecule delivery.
研究不足
The injection process requires precise control of laser power to avoid cell damage. The technique is limited by the size of the nanopens and the resistance provided by the cell's cytoskeleton.
1：Experimental Design and Method Selection:
The study involves the use of plasmonic Janus nanopens made of a gold nanoparticle attached to a dielectric alumina shaft. The nanopens are trapped and positioned on the surface of living cells using an optical tweezer, balancing optical and thermophoretic forces.
2：Sample Selection and Data Sources:
Living cells and Janus nanopens functionalized with fluorescently labelled oligo-ssDNA are used.
3：List of Experimental Equipment and Materials:
Optical tweezer, near-infrared (NIR) laser, green laser, dark-field microscope, fluorescence microscope, scanning electron microscope (SEM).
4：Experimental Procedures and Operational Workflow:
Single JNPs are trapped and positioned on the cell surface using a NIR laser. Injection is performed with a green laser close to the plasmon resonance. The process is monitored using dark-field and fluorescence microscopy.
5：Data Analysis Methods:
The displacement of JNPs in the optical trap is analyzed based on the diffraction pattern. Temperature distribution around the JNPs is simulated.
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