研究目的
To develop a practical undergraduate laboratory experiment for teaching FRET using a diverse set of green-emitting ?uorescent proteins (FPs) as donors for a cross-linked Yukon orange FP, enabling students to connect basic lab procedures to real-world applications.
研究成果
The experiment successfully highlights important properties of GFP-based FRET, extending the use of GFPs in undergraduate laboratory experiments to a new topic. It develops students' basic laboratory biochemistry skills and provides hands-on experience with current research involving GFP fusion for FRET, suitable for upper-level undergraduate and graduate-level laboratory courses.
研究不足
The complexity and difficulty of constructing FRET experiments limit their usage in undergraduate laboratory settings. The experiment requires access to specific equipment and materials, and students may need more time to hone their techniques and understand the theory fully.
