研究目的
Investigating the use of molecular recognition force spectroscopy for the characterization and optimization of targeted nanoparticles to achieve cell-specific interaction.
研究成果
The use of MRFS allowed for the determination of the optimal targeted nanoparticle formulation regarding neuronal cell internalization and transfection, as well as provided new insights into the ligand-receptor mechanism. The results illustrate the potential of this novel approach for the rapid and cost-effective design of targeted nanoparticle systems, as MRFS has proved to be a reliable method that allows reproducible and comparable results independently of the biological model being tested.
研究不足
The study acknowledges that MRFS experiments are relatively straightforward to set up but highlights critical steps such as sample preparation, AFM tip functionalization, and data evaluation as paramount for obtaining suitable data. The need for careful selection of AFM probes, functionalization chemistry, and buffer conditions is emphasized to ensure the binding activity of the molecule is not altered.
1：Experimental Design and Method Selection:
The study employs molecular recognition force spectroscopy (MRFS) to characterize and optimize targeted nanoparticles. The methodology involves the use of atomic force microscopy (AFM) to explore the forces and dynamics of the interaction between individual ligands and receptors on cellular surfaces.
2：Sample Selection and Data Sources:
ND7/23 cells (mouse neuroblastoma and rat dorsal root ganglion neuron hybrid cell line) and NIH 3T3 cells (mouse embryonic fibroblasts) were used as sample and control cell lines, respectively.
3：List of Experimental Equipment and Materials:
Includes AFM setup (PicoPlus 5500 AFM setup and fluid cell equipped with flow cell from Agilent Technologies), silicon nitride AFM cantilevers (MSCT, Bruker), and various chemicals for nanoparticle preparation and tip functionalization.
4：Experimental Procedures and Operational Workflow:
The protocol involves cell sample preparation, nanoparticle preparation, AFM tip functionalization, force measurements, and data analysis to determine the specificity of the functionalized AFM tip.
5：Data Analysis Methods:
Numerical calculations were performed using MATLAB? Version R2009b (MATH WORKS Inc.) to find the position of unbinding events and calculate the respective unbinding forces.
独家科研数据包，助您复现前沿成果，加速创新突破
获取完整内容
