研究目的
Investigating the emergence of the protein-like fluorophore as a result of DOM phototransformation in natural waters.
研究成果
The study demonstrates that the increase in protein-like fluorescence of natural waters may result from phototransformation of DOM, not just microbial production. The phototransformation is a two-step process involving degradation during irradiation and partial regeneration during dark storage, with the most prominent recovery observed in the protein-like fluorophore spectral range.
研究不足
The study focuses on the phototransformation of DOM in natural waters but does not extensively explore the microbial contributions to the protein-like fluorophore. The experimental conditions, such as the use of UV radiation and dark storage, may not fully replicate natural environmental conditions.
1：Experimental Design and Method Selection:
Samples of natural waters were irradiated using ultraviolet radiation in the solar wavelength range. Their EMM spectra were recorded before and directly after irradiation, and after 72 h long dark storage.
2：Sample Selection and Data Sources:
River water was collected at the Vistula River outlet, and samples of surface coastal and sea water were taken in summer
3：List of Experimental Equipment and Materials:
20 Quartz tubes, Cleo Advantage fluorescent tubes, SKU 430 UVB and SKU 421 UVA broadband sensors, Vario Cube TOC analyzer, Jasco 630 V dual-beam spectrophotometer, Jasco FP6300 spectrofluorometer.
4：Experimental Procedures and Operational Workflow:
Samples were filtered, irradiated for 20 h, and then stored in dark for 72 h. Fluorescence and absorbance measurements were taken before and after irradiation and after dark storage.
5：Data Analysis Methods:
Fluorescence values were normalized to Raman units, and second order Rayleigh scattering and Raman scattering were deleted using data conversion.
独家科研数据包，助您复现前沿成果，加速创新突破
获取完整内容
