研究目的
To address the limitations of current optical imaging techniques for lymph node mapping, such as poor tissue penetration and auto-fluorescence effect, by developing a novel three-dimensional optical molecular imaging technique named radiopharmaceutical-excited fluorescence imaging (REFI).
研究成果
The study demonstrates that REFI can significantly enhance the light intensity and accuracy of three-dimensional imaging of lymph nodes, with location deviations less than 1.03 mm, by combining radioisotope-excited fluorescence with advanced reconstruction algorithms.
研究不足
The study is limited by the inherent challenges of optical imaging in biological tissues, such as scattering and limited boundary measurements, which affect the accuracy of 3D reconstruction.
1：Experimental Design and Method Selection:
The study combines the merits of Cerenkov luminescence imaging (CLI) and fluorescence molecular imaging (FMI) to develop REFI, using gamma-ray and Cerenkov radiation from radioisotopes to excite lanthanide europium oxide (EO) nanophosphors.
2：Sample Selection and Data Sources:
EO nanoparticles were purchased and their excitation and emission profiles were measured. Mice models were used for in vivo imaging.
3：List of Experimental Equipment and Materials:
A fluorospectrophotometer (Hitachi, FL9000) and an in vivo imaging system (IVIS) (Caliper Life Sciences) were used.
4：Experimental Procedures and Operational Workflow:
The study involved spectrophotometry of EO, comparison of REFI and CLI signals, and 3D reconstruction of lymph nodes using an ASD-POCS algorithm.
5：Data Analysis Methods:
The light transport in biological tissue was modeled using the diffusion equation, and the ASD-POCS algorithm was used for 3D reconstruction.
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