研究目的
Investigating the effects of a Glu364 to Gln substitution in the CP47 protein and external pH on the stability of redox-active TyrD and charge recombination in Photosystem II.
研究成果
The Glu364 to Gln substitution in CP47 affects the stability of YD and the redox state of the OEC, with external pH influencing photosynthetic electron transfer. The study suggests that the hydrogen bond network around YD is crucial for PS II function and that environmental pH can modulate electron transfer processes in intact cyanobacterial cells.
研究不足
The study is limited to the cyanobacterium Synechocystis sp. PCC 6803 and may not directly apply to other organisms. The effects of external pH on thylakoid lumen pH are inferred rather than directly measured.
1：Experimental Design and Method Selection:
The study used thermoluminescence, chlorophyll fluorescence, and flash-induced oxygen evolution analyses to assess the effects of the Glu364 to Gln mutation and pH on Photosystem II activity.
2：Sample Selection and Data Sources:
Synechocystis sp. PCC 6803 strains, including wild type and mutants (E364Q, ?PsbV, E364Q:?PsbV), were grown under photoautotrophic conditions at pH
3：5 and pH List of Experimental Equipment and Materials:
Custom-built thermoluminescence machine, Joliot-type electrode for oxygen evolution, FL-3000 double-modulation fluorimeter for chlorophyll fluorescence, and Dual-PAM-100 measuring system for P700 oxidation state.
4：Experimental Procedures and Operational Workflow:
Cells were subjected to specific illumination and dark adaptation periods before measurements. TL was measured after cooling and warming samples, oxygen evolution was measured following dark adaptation and actinic flashes, and fluorescence decay was measured after single-turnover flashes.
5：Data Analysis Methods:
Data were analyzed using custom-made software for TL, Origin 2017 for fluorescence decay, and matrix formalism of the Joliot-Kok model for oxygen evolution parameters.
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