研究目的
Investigating a low-cost, readily available method for retrofitting microscopy imaging systems to achieve 3D focus scanning.
研究成果
The proposed low-cost method for retrofitting imaging systems enables 3D focus scanning and provides all-in-focus, high-resolution images as well as corresponding depth maps. The system's performance could be improved with a more detailed thickness distribution and higher update frequency.
研究不足
The addition of dissimilar plates could increase chromatic aberration and decrease resolution. Mechanical errors could introduce spherical aberration and prism-like eccentricity error.
1：Experimental Design and Method Selection:
The study involved designing a variable focus spinner with dissimilar plates to achieve 3D focus scanning.
2：Sample Selection and Data Sources:
A target (Arduino Mini chip) was placed at 45? with respect to the optical axis and observed using a microscopy lens kit.
3：List of Experimental Equipment and Materials:
Included a microlens (EO-55-359, Edmund Optics), a high-speed camera (acA1300-200uc, Basler), and transparent plates with various thicknesses.
4：Experimental Procedures and Operational Workflow:
A sequence of 12 images was captured in different focal planes by rotating the spinner.
5：Data Analysis Methods:
Image scale and phase correction were performed, and in-focus pixels were abstracted using the Laplacian operator to generate an all-in-focus image and a depth map.
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