研究目的
Investigating the spectral stability of small-molecule fluorescent probes under high (de)excitation light intensities for multicolor fluorescence imaging and single-molecule applications.
研究成果
The study successfully identified substitution patterns that provide photoconversion-resistant triarylmethane fluorophores, demonstrating their applicability in live-cell STED nanoscopy. The findings emphasize the importance of spectral stability for accurate multicolor fluorescence imaging.
研究不足
The study focuses on triarylmethane fluorophores and their photostability under specific conditions. The applicability of findings to other fluorophore classes or under different environmental conditions may require further investigation.
