研究目的
To assess the reliability of indices derived from imaging techniques—thermal indices (Ig and CWSI) and optical indices (NDVI and PRI)—as operational tools to detect the crop water status, regardless the eventual presence of nitrogen stress.
研究成果
The study confirmed that imaging indices such as CWSI, NDVI and PRI can be used as operational tools to predict soil water status and to detect drought stress under different soil nitrogen conditions. Thermal indices derived from high resolution thermal images (especially CWSI) can be seen as an adequate operational tool to monitor the crop water status, since no dependence on plant nitrogen conditions was observed.
研究不足
The study was conducted in a greenhouse, which may have some limits in different seasons, such as low light intensity during winter, and a general tendency to high air relative humidity (low vapor pressure deficit (VPD)) which limits leaf gas exchange.
1：Experimental Design and Method Selection:
Two separate experiments were carried out in a greenhouse, on two spinach varieties (Verdi F1 and SV2157VB), with different microclimatic conditions and under different levels of water and nitrogen application.
2：Sample Selection and Data Sources:
Two spinach varieties were selected for the experiments.
3：List of Experimental Equipment and Materials:
Thermal infrared camera (TVS-200EX NEC Avio Infrared Technologies Co. Ltd.), hyperspectral imaging line scan sensor (Specim V10 spectrometer and a Basler PiA190032gm sensor), weighing balance, porometer (LICOR-1600), fluorimeter (Handy-PEA), and others.
4：Experimental Procedures and Operational Workflow:
Thermal and hyperspectral images were acquired, and reference measurements (stomatal conductance, chlorophyll a fluorescence, and leaf and soil water content) were taken.
5：Data Analysis Methods:
Statistical analysis based on ANOVA test, correlation analysis, and linear regression models were used.
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