研究目的
To develop a novel near infrared (NIR) activated surface-enhanced Raman scattering (SERS) nanosensor for in-vitro and in-vivo hydrogen sulfide (H2S) assay.
研究成果
The developed Au/Ag nanoboxes based NIR SERS system is highly sensitive, selective, and rapid for H2S detection in bio-objects. It can monitor H2S distribution in living cells and zebrafish embryos with a detection limit of 10 nM and a penetration depth of up to 12 mm in tissues, demonstrating its potential for in-vivo studies of pathophysiological processes involving H2S.
研究不足
The study focuses on the detection of H2S in controlled environments (in-vitro and in-vivo models). The practical application in complex biological systems may require further optimization.
1：Experimental Design and Method Selection:
The study utilized Au/Ag alloy nanoboxes with local surface plasmon resonance (LSPR) at 741 nm as the SERS substrate. NIR probes (3,3'-diethylthiatricarbocyanine iodide, DTTC) were labeled on Au/Ag nanoboxes for H2S detection.
2：Sample Selection and Data Sources:
HeLa cells and zebrafish embryos were used as biological specimens for in-vitro and in-vivo H2S detection, respectively.
3：List of Experimental Equipment and Materials:
Au/Ag alloy nanoboxes, DTTC NIR probes, NIR laser (785 nm), Raman microscope.
4：Experimental Procedures and Operational Workflow:
Au/Ag nanoboxes were synthesized and labeled with DTTC. The SERS spectra were collected under NIR laser irradiation before and after the introduction of H2S.
5：2S. Data Analysis Methods:
5. Data Analysis Methods: The variation of SERS peak intensities was analyzed to determine H2S concentrations.
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