研究目的
Investigating the therapeutic effects of a specific DNA aptamer (R13) on ovarian cancer diagnosis and treatment.
研究成果
The R13 aptamer shows high binding affinity and specificity for ovarian cancer cells, targets membrane proteins, and is internalized via clathrin- and caveolae-mediated pathways. It holds promise as a novel tool for ovarian cancer diagnosis and targeted drug delivery.
研究不足
The study did not identify the specific membrane proteins targeted by R13. The potential nonspecific toxic effects due to liver or kidney uptake of the aptamer were not fully addressed.
1：Experimental Design and Method Selection:
The study involved flow cytometry analysis, confocal microscope imaging, and in vivo tumor models to assess the binding ability, stability, and internalization of the R13 aptamer.
2：Sample Selection and Data Sources:
Ovarian cancer cell lines (HO8910, Caov3, A2780, SKOV3), ovarian cancer tissues, normal ovary tissues, and fallopian tube tissues were used.
3：List of Experimental Equipment and Materials:
Flow cytometer, confocal microscope, tissue microarray, and various buffers and reagents were utilized.
4：Experimental Procedures and Operational Workflow:
Cells were incubated with R13, and binding affinity was measured. Tissue sections were stained with R13, and fluorescence images were obtained. In vivo imaging was performed on nude mouse xenograft tumor models.
5：Data Analysis Methods:
The equilibrium dissociation constants (Kd) were calculated using GraphPad Prism5. Fluorescence intensity was analyzed with Quant Center.
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